| 产品名称: |
Recombinant Mouse Beta-NGF (110AA) |
| 产品形貌: |
Recombinant Mouse Beta-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Met130-Arg239 is expressed. |
| 卵白编号: |
P01139 |
| 分子量: |
12.4 KDa |
| 表观分子量: |
12 KDa, reducing conditions |
| 产品纯度: |
Greater than 95% as determined by reducing SDS-PAGE. |
| 内毒素: |
Less than 0.1 ng/?g (1 EU/?g) as determined by LAL test. |
| 生物活性: |
Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 68.52 ng/ml. |
| 复溶: |
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.? |
| 贮存条件: |
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| 运输条件: |
The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below. |
| 研究配景: |
NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF. NGF signaling has been shown to play an important role in neuroprotection and repair. β-NGF acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. It is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. |
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