| 产品名称: |
Recombinant K. pneumoniae NEO |
| 产品形貌: |
Recombinant Klebsiella Pneumoniae Aminoglycoside 3'-phosphotransferase is produced by our E.coli expression system and the target gene encoding Met1-Phe264 is expressed. |
| 卵白编号: |
P00552 |
| 分子量: |
29 KDa |
| 表观分子量: |
26-30 KDa, reducing conditions |
| 产品纯度: |
Greater than 95% as determined by reducing SDS-PAGE. |
| 内毒素: |
Less than 0.1 ng/?g (1 EU/?g) as determined by LAL test. |
| 贮存条件: |
Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening.
Please minimize freeze-thaw cycles. |
| 运输条件: |
The product is shipped on dry ice/polar packs.
Upon receipt, store it immediately at the temperature listed below. |
| 研究配景: |
Aminoglycoside 3'-phosphotransferase (APH(3')), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3'-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH(3') is primarily found in certain species of gram-positive bacteria. |
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