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大鼠α-血红卵白稳固卵白(aHSP)酶联免疫吸附检测试剂盒

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ELK7701

规格：	价钱：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下载说明 ①

Overview

产品名称:	Rat aHSP(Alpha-Hemoglobin Stabilizing Protein) ELISA Kit
反应性:	Rat
又名:	A-HSP; ERAF; EDRF; Erythroid Associated Factor; Erythroid differentiation-related factor
反应类型:	Sandwich
迅速度:	0.56 ng/mL
标准品:	100 ng/mL
检测规模:	1.57-100 ng/mL
样本类型:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
反应时长:	3.5h
研究领域:	Metabolic pathway;Infection immunity;Cardiovascular biology;
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat aHSP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat aHSP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat aHSP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat aHSP in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
100.00	2.132	2.048
50.00	1.609	1.525
25.00	1.106	1.022
12.50	0.928	0.844
6.25	0.593	0.509
3.13	0.357	0.273
1.57	0.228	0.144
0.00	0.084	0.000

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细密度

板内细密度（测定内的细密度）：CV%<8%

三个已知浓度的样品划分在1个酶标板上测试20次，，，以评估剖析板内细密度。。。。

板间细密度（测定板间间细密度）：CV%<10%

三个已知浓度的样品划分在3个差别的酶标板上测试40次，，，以评估剖析板间的细密度。。。。

接纳率

划分往差别样本中添加已知浓度的aHSP，，，做接纳率实验，，，得出接纳率规模清静均接纳率

样本类型	接纳率规模	平均接纳率
血清(n=5)	95-108%	101%
EDTA 血浆(n=5)	83-97%	90%
Heparin 血浆(n=5)	80-97%	88%

线性

将添加有aHSP的样天职别稀释2倍，，，4倍，，，8倍，，，16倍做接纳实验，，，得出接纳率规模

样本类型	1:2	1:4	1:8	1:16
血清(n=5)	85-97%	87-96%	85-96%	85-95%
EDTA 血浆(n=5)	95-106%	87-96%	82-90%	92-101%
Heparin 血浆(n=5)	85-92%	95-103%	97-105%	93-102%

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