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大鼠O-6-甲基鸟嘌呤DNA甲基转移酶(MGMT)酶联免疫吸附检测试剂盒

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ELK7160

规格：	价钱：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下载说明 ①

Overview

产品名称:	Rat MGMT(O-6-Methylguanine DNA Methyltransferase) ELISA Kit
反应性:	Rat
又名:	Methylated-DNA--Protein-Cysteine Methyltransferase; 6-O-methylguanine-DNA methyltransferase; Methylated-DNA--protein-cysteine methyltransferase
反应类型:	Sandwich
迅速度:	27 pg/mL
标准品:	5000 pg/mL
检测规模:	78.13-5000 pg/mL
样本类型:	Tissue homogenates and other biological fluids.
反应时长:	3.5h
研究领域:	Signal transduction;Enzyme & Kinase;Tumor immunity;
Uniprot ID:	P24528
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MGMT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MGMT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MGMT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MGMT in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.163	2.095
2500.00	1.484	1.416
1250.00	0.885	0.817
625.00	0.665	0.597
312.50	0.493	0.425
156.25	0.312	0.244
78.13	0.206	0.138
0.00	0.068	0.000

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细密度

板内细密度（测定内的细密度）：CV%<8%

三个已知浓度的样品划分在1个酶标板上测试20次，，以评估剖析板内细密度。。。。

板间细密度（测定板间间细密度）：CV%<10%

三个已知浓度的样品划分在3个差别的酶标板上测试40次，，以评估剖析板间的细密度。。。。

接纳率

划分往差别样本中添加已知浓度的MGMT，，做接纳率实验，，得出接纳率规模清静均接纳率

样本类型	接纳率规模	平均接纳率
血清(n=5)	90-104%	97%
EDTA 血浆(n=5)	80-97%	88%
Heparin 血浆(n=5)	87-99%	93%

线性

将添加有MGMT的样天职别稀释2倍，，4倍，，8倍，，16倍做接纳实验，，得出接纳率规模

样本类型	1:2	1:4	1:8	1:16
血清(n=5)	87-98%	87-100%	85-94%	83-96%
EDTA 血浆(n=5)	85-100%	84-98%	95-101%	88-102%
Heparin 血浆(n=5)	79-92%	85-96%	80-98%	95-102%

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