小鼠肿瘤坏死因子α(TNFa)酶联免疫吸附检测试剂盒-ELK意大利贵宾生物-ELISA试剂盒、抗体、卵白、分子

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小鼠肿瘤坏死因子α(TNFa)酶联免疫吸附检测试剂盒

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ELK1387

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48T	￥1680.00
96T	￥2400.00
96T*5	￥10200.00

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产品名称:	Mouse TNFa(Tumor Necrosis Factor Alpha) ELISA Kit
反应性:	Mouse
又名:	TNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
反应类型:	Sandwich
迅速度:	6.2 pg/mL
标准品:	1000 pg/mL
检测规模:	15.63-1000 pg/mL
样本类型:	血清、血浆、组织匀浆、细胞裂解液、细胞作育上清液和其他生物体液
反应时长:	3.5h
研究领域:	细胞因子;肿瘤免疫;熏染免疫学;
Uniprot ID:	P06804
检测原理:	本试剂盒接纳夹心法原理。。。将特异性抗小鼠 TNFa抗体包被于96孔微孔板中，，，，向微孔中划分加入小鼠 TNFa标准品或样本，，，，使标准品中的小鼠 TNFa卵白或样本中的小鼠 TNFa卵白与固相于微孔板上的抗小鼠 TNFa抗体连系，，，，然后加入生物素化抗小鼠 TNFa抗体，，，，将未连系的生物素化抗体洗净后，，，，加入HRP标记链亲和素，，，，再次彻底洗涤后加入TMB底物显色。。。TMB在过氧化物酶催化下转化成蓝色，，，，并在酸的作用下转化成最终的黄色。。。颜色的深浅和样品中的小鼠 TNFa卵白呈正相关。。。用酶标仪在450 nm波长下测定吸光度(OD值)，，，，通过绘制标准曲线盘算出样品浓度。。。

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