人肿瘤坏死因子α(TNFa)酶联免疫吸附检测试剂盒-ELK意大利贵宾生物-ELISA试剂盒、抗体、卵白、分子

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人肿瘤坏死因子α(TNFa)酶联免疫吸附检测试剂盒

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ELK1190

规格：	价钱：
48T	￥1680.00
96T	￥2400.00
96T*5	￥10200.00

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Overview 文献(60)

产品名称:	Human TNFa(Tumor Necrosis Factor Alpha) ELISA Kit
反应性:	Human
又名:	TNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
反应类型:	Sandwich
迅速度:	6.4 pg/mL
标准品:	1000 pg/mL
检测规模:	15.63-1000 pg/mL
样本类型:	血清、血浆、组织匀浆、细胞裂解液、细胞作育上清液和其他生物体液
反应时长:	3.5h
研究领域:	细胞因子;肿瘤免疫;熏染免疫学;
Uniprot ID:	P01375
检测原理:	本试剂盒接纳夹心法原理。。。。。将特异性抗人 TNFa抗体包被于96孔微孔板中，，，，，向微孔中划分加入人 TNFa标准品或样本，，，，，使标准品中的人 TNFa卵白或样本中的人 TNFa卵白与固相于微孔板上的抗人 TNFa抗体连系，，，，，然后加入生物素化抗人 TNFa抗体，，，，，将未连系的生物素化抗体洗净后，，，，，加入HRP标记链亲和素，，，，，再次彻底洗涤后加入TMB底物显色。。。。。TMB在过氧化物酶催化下转化成蓝色，，，，，并在酸的作用下转化成最终的黄色。。。。。颜色的深浅和样品中的人 TNFa卵白呈正相关。。。。。用酶标仪在450 nm波长下测定吸光度(OD值)，，，，，通过绘制标准曲线盘算出样品浓度。。。。。

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